C2181

抗マウスIgG (全分子) F(ab′)₂ フラグメント–Cy3 ヒツジ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: Cy3 Anti-Mouse IgG, Cy3 Mouse IgG, Sheep Anti-Mouse IgG
• MDL番号: MFCD02179333
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: sheep
• 品質水準: 200
• 結合体: CY3 conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 化学種の反応性: mouse
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Igs have two heavy (H) and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.

アプリケーション

全てのマウスイムノグロブリンに結合します。
Fcレセプタ-存在下のバックグラウンド染色を避ける際に用いられます。

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Cy3 antibody produced in sheep was used for BrdU staining of frozen quail muscle cross sections at a dilution of 1:200 to analyze activated, proliferating muscle precursor cells.

Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment−Cy3 antibody produced in sheep has been used:

• in immunolabeling of Hela cells
• as secondary antibody in immunocytochemistry of dendritic cells
• as secondary antibody in immunofluorescence analysis of mesenchymal stem cells
• as secondary antibody in immunofluorescence staining keratinocyte cell lines
• in immunohistochemistry of articular cartilage

生物化学的／生理学的作用

Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Mouse IgG (whole molecule) F(ab′)2 fragment antibody allows for the visualization of protein by fluorescent microscopy.

その他情報

本抗体は、ヒト血清タンパク質で吸収済です。

物理的形状

0.01 M PBS溶液(pH 7.4, 1% BSA, 15 mMアジ化ナトリウム含有)。

調製ノート

ヒトサンプルでのバックグラウンド染色の低減のため吸収処理済み。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable