I2513

モノクロナール抗ヒトIgG1 マウス宿主抗体

clone 8c/6-39, ascites fluid

• 別名: モノクロナール抗ヒトIgG1 (Fc特異的)
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: secondary antibodies
• クローン: 8c/6-39, monoclonal
• 含みます: 15 mM sodium azide
• テクニック: indirect ELISA: 1:400
• アイソタイプ: IgG2a
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

この抗体はヒトIgG1のFc部位に特異的で他のIgGサブクラスとは反応しません。このクローンは、HP-6091とも記述され、IUIS/WHOの研究によってヒトIgG1で反応性と特異性の有益な側面を示すことが見出されました。

Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Human IgG consists of four subclasses (1-4) that can be recognized by antigen differences in their heavy chains. They constitute approximately 65, 25, 6 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties.

The IgG subclass may be preferentially produced in response to different antigens. For instance, antipolysaccharide responses are mainly of the IgG2 subclass, while protein antigens give rise to IgG1 and IgG3 antibodies. Lipopolysaccahrides stimulate an IgG2 response in PBL′s and an IgG1 response in the spleen. Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, IgG2 and IgG4 deficiency is associated with IgA deficiency as found in patients of ataxia telangiextasia. Low IgG2 levels were found in patients with SLE and juvenile diabetes melitus. A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and may assist in the diagnosis of various disorders.

特異性

Human IgG consists of four subclasses (1-4) that can be recognized.

アプリケーション

Monoclonal Anti-Human IgG1 antibody produced in mouse has been used in:

• direct hemagglutination(HA)
• hemagglutination inhibition(HAI) assays
• enzyme linked immunosorbent assay (ELISA)
• immunofluorometric assay (IFMA)
• immunofluorescence
• cell-based assays for the detection of IgG subclasses of acetylcholine receptor (AChR) antibodies

Mouse monoclonal clone clone 8c/6-39 anti-Human IgG1 antibody may be used for the identification of human IgG1 subclass by means of various immunoassays and direct hemagglutination (HA) and hemagglutination inhibition (HAI) assays, enzyme linked immunosorbent assay (ELISA), immunofluorometric assay (IFMA) and detection of cytoplasmic IgG. The antibody may be useful in the analysis of the distribution of IgG subclasses in various diseases.

生物化学的／生理学的作用

Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes via Fc receptors (FcR). A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis.

物理的形状

The antibody is provided as ascites fluid with 0.1% sodium azide as a preservative.

保管および安定性

For continuous use, store at 0-5 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable