L6385

α-ラクトアルブミン from bovine milk

For use as a marker in SDS-PAGE

• 別名: alpha-lactalbumin
• CAS番号: 9051-29-0
• MDL番号: MFCD00162025
• UNSPSCコード: 12352202
• NACRES: NA.61

特性

• 由来生物: bovine milk
• 品質水準: 200
• 形状: powder
• 分子量: ~14.2 kDa
• 包装: vial of 5 mg
• テクニック: electrophoresis: suitable
• 溶解性: H₂O: soluble 10 mg/mL
• UniProtアクセッション番号: P00711
• 保管温度: 2-8°C
• 遺伝子情報: cow ... LALBA(281894)

詳細

詳細

α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands.

アプリケーション

α-Lactalbumin from bovine milk is suitable for use:

• as an electrophoresis marker, with a molar mass of approximately 14,200Da
• in a study to investigate selective binding of proteins on charged surface iron oxide nanoparticles via reverse charge parity model

α-Lactalbumin was used in the reduction of the antigenicity of whey proteins by lactic acid fermentation.

生物化学的／生理学的作用

ガラクトシルトランスフェラーゼの基質特異性を変化させてラクトース生成速度を上昇させます。ガラクトシルトランスフェラーゼとα-ラクトアルブミンの複合体はラクトースシンターゼとして知られています。Asp⁸⁷またはAsp⁸⁸のAlaへの部位特異的突然変異誘発は、強力なカルシウム結合親和性を完全に無効にし、ラクトースシンターゼの刺激を最大速度の3.5%未満に低下させます。

α-Lactalbumin consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form. α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Angstrom resolution, demonstrating four a-helices and a triple stranded antiparallel β-sheet.