L4385

α-ラクトアルブミン from bovine milk

Marker for non-denaturing PAGE

• CAS番号: 9051-29-0
• EC Number: 235-747-3
• MDL番号: MFCD00162025
• UNSPSCコード: 12352202
• NACRES: NA.61

特性

• 由来生物: bovine milk
• 品質水準: 200
• 形状: lyophilized powder
• 分子量: ~14.2 kDa
• テクニック: microbiological culture: suitable
• UniProtアクセッション番号: P00711
• 保管温度: −20°C
• 遺伝子情報: cow ... LALBA(281894)

詳細

詳細

α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands.

アプリケーション

α-Lactalbumin was used in the isolation and analysis of restriction endonuclease digestive patterns of chromosomal DNA from Mycobacterium paratuberculosis and other Mycobacterium species. It was also used in a study to test if the neuroendocrine protein 7B2 suppresses the aggregation of neurodegenerative disease-related proteins.

生物化学的／生理学的作用

ガラクトシルトランスフェラーゼの基質特異性を変化させてラクトース生成速度を上昇させます。ガラクトシルトランスフェラーゼとα-ラクトアルブミンの複合体はラクトースシンターゼとして知られています。Asp⁸⁷またはAsp⁸⁸のAlaへの部位特異的突然変異誘発は、強力なカルシウム結合親和性を完全に無効にし、ラクトースシンターゼの刺激を最大速度の3.5%未満に低下させます。

α-Lactalbumin is the cheif protein in human milk. It consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form. α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Α° resolution, demonstrating four α-helices and a triple stranded antiparallel β-sheet.

再構成

1 mLの水で再構成後、0.9~1.4 mg/mL

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable