N6786

モノクロナール抗Notch1

clone mN1A, purified from hybridoma cell culture

• 別名: Notch antibody
• MDL番号: MFCD03455659
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: mN1A, monoclonal
• 形状: buffered aqueous solution
• 化学種の反応性: mouse, human
• テクニック: immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using extracts of cells stably transfected with a truncated Notch1 (32D-mN1-ICDOP that expresses the intracellular mouse Notch1 molecule-65 kDa).
• アイソタイプ: IgG1
• UniProtアクセッション番号: P46531
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NOTCH1(4851) ; mouse ... Notch1(18128)

詳細

詳細

ヒト・マウスの開裂したNotch1 (約300 kDa, 120 kDa) を認識します。本抗体は、Notch2, 3, 4を認識しません。 細胞内活性化型Notchに対し高い親和性を示します。

The Notch protein family is a group of highly conserved proteins important in the determination of cell fate and maintenance of progenitors in many developmental systems.In mammals, four Notch genes were identified (Notch1-4) in a wide variety of cells and play a crucial role in differentiation and development. Notch proteins contain three main domains: an extracellular domain containing tandem epidermal growth factor repeats, and three lin-12/Notch repeats, a transmembrane domain, and an intracellular domainthat contains six cdc1-/SW16/ankyrin repeats, nuclear localization signals, and a C-terminal OPA/PEST region.

特異性

ヒト・マウスの開裂したNotch1 (約300 kDa, 120 kDa) を認識します。本抗体は、Notch2, 3, 4を認識しません。 細胞内活性化型Notchに対し高い親和性を示します。

免疫原

マウスNotch1のcdc10-NCR領域を含むリコンビナントタンパク質。

アプリケーション

Anti-Notch1 antibody, mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• immunohistochemistry

生物化学的／生理学的作用

NOTCH is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism. Fibronectin functions both as a regulator of cellular processes and as an important scaffolding protein to maintain and direct tissue organization and ECM composition.Fibronectin is suggested to enhance cell adhesion, spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose most of their surface bound fibronectin.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)