N9657

モノクローナル抗NOシンターゼ, 誘導型抗体 マウス宿主抗体

clone NOS-IN, ascites fluid

• 別名: 抗iNOS抗体
• MDL番号: MFCD00675308
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: NOS-IN, monoclonal
• 分子量: antigen 130 kDa
• 含みます: 15 mM sodium azide
• 化学種の反応性: mouse, rat
• 包装: antibody small pack of 25 μL
• テクニック: microarray: suitable; western blot: 1:1,000 using whole cell extract of the murine macrophage cell line RAW 264.7 activated with LPS and IFN-γ
• アイソタイプ: IgG1
• UniProtアクセッション番号: Q06518
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NOS2(4843) ; mouse ... Nos2(18126) ; rat ... Nos2(24599)

詳細

詳細

Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS) (mouse IgG1 isotype) is derived from the NOS-IN hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. Nitric oxide synthase (NOS) is classified into three types based on molecular mass, subcellular location, and Ca²⁺ dependence. Type I NOS (150-160 kDa) is found in neurons. It is also called as NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca²⁺-regulated NOS (cNOS). Type II (130 kDa), best characterized in macrophages, also known as macrophage NOS (mNOS) or inducible NOS (iNOS). Type III (135 kDa) is found in endothelial cells. It is called as endothelial NOS (eNOS, or ecNOS). Type II NOS is Ca²⁺ -independent and is expressed in activated macrophages and some glial cells after stimulation. iNOS is also found in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts.

特異性

Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS), antibody reacts specifically with inducible-type nitricoxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain.

免疫原

マウスマクロファ-ジ由来NOシンタ-ゼ(iNOSまたはmacNOS)のアミノ酸1126-1144に相当する合成ペプチドのKLH結合体。免疫原の配列はラットiNOSで高度に保存されていますが、ヒトiNOSとは異なっています。

アプリケーション

Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS) has been used in

• immunofluorescence staining
• immunogold electron microscopy
• immunoblotting
• immunohistochemistry
• immunocytochemistry
• enzyme linked immunosorbent assay (ELISA)

生物化学的／生理学的作用

Nitric oxide synthase (NOS) is an enzyme involved in the synthesis of nitric oxide (NO), a free radical generated under physiological conditions by virtually all mammalian cells. NO is formed from arginine by NOS which oxidizes a guanidino nitrogen of arginine, releasing NO and citrulline. NO is a messenger molecule mediating diverse functions including vasodilatation, neurotransmission, and antimicrobial and anti-tumor activities. Various types of NOS may serve a variety of diverse biological pathways.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable