P0089

Anti-Prox1 antibody , Mouse monoclonal

clone 4G10, purified from hybridoma cell culture

• 別名: Anti-Homeobox prospero-like protein Prox1, Anti-Prospero homeobox 1
• MDL番号: MFCD09039204
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ELISA (i); ICC; IF; WB
• クローン: 4G10, monoclonal
• 化学種の反応性: chicken, rat, lizard, human
• citations: 6
• テクニック: immunocytochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HepG2 cells; western blot: suitable

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 4G10, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~84 kDa
• 化学種の反応性: chicken, rat, lizard, human
• 濃度: ~1.5 mg/mL
• テクニック: immunocytochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HepG2 cells; western blot: suitable
• アイソタイプ: IgG2b
• UniProtアクセッション番号: Q92786
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PROX1(5629) ; rat ... Prox1(305066)

詳細

詳細

Monoclonal Anti-Prox1 (mouse IgG2b isotype) is derived from the hybridoma 4G10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein of human Prox1. Prospero related homeobox gene 1 (Prox1) belongs to transcription factor subfamily and is homologous to Drosophila homeobox protein, prospero.

免疫原

recombinant fusion protein corresponding to amino acids 547-737 of human Prox1.

アプリケーション

Anti-Prox1 antibody, Mouse monoclonal has been used in various immunochemical techniques.

生物化学的／生理学的作用

Prospero related homeobox gene 1 (Prox1) is a key regulator for neuroblast fate determination and photoreceptor development. Prox1 regulates cell differentiation and organogenesis in lens, retina, lymphatic vessels, liver and pancreas. It was found to act as a tumor suppressor gene, because its deficiency or its mutated forms have been implicated in various human cancers. Knockdown of Prox1 by siRNA or by RNA mutations significantly accelerated the in vitro and in vivo growth of hepatocellular carcinoma (HCC) as well as esophageal cancer cells. Prox1 is hypermethylated and transcriptionally silenced in primary and metastatic breast cancer.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)