P2860

モノクロナール抗PSF マウス宿主抗体

clone B92, purified from hybridoma cell culture

• 別名: Mouse Anti-Polypyrimidine tract-binding protein-associated splicing factor
• MDL番号: MFCD03457573
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ARR; ICC; IHC (f); IP; RIA; WB
• クローン: B92, monoclonal
• 化学種の反応性: mouse, human
• citations: 37
• テクニック: immunocytochemistry: suitable; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; microarray: suitable; radioimmunoassay: suitable using cells; western blot: 2-4 μg/mL using whole extract of cultured HeLa cells

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: B92, monoclonal
• フォーム: buffered aqueous solution
• 化学種の反応性: mouse, human
• 包装: antibody small pack of 25 μL
• 濃度: ~2 mg/mL
• テクニック: immunocytochemistry: suitable; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; microarray: suitable; radioimmunoassay: suitable using cells; western blot: 2-4 μg/mL using whole extract of cultured HeLa cells
• アイソタイプ: IgG1
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TAP1(6890)

詳細

詳細

Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.

免疫原

マウス骨髄由来間質細胞株のライセ-ト。

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)

Monoclonal Anti-PSF antibody produced in mouse has been used in:

• immunofluorescence
• immunohistochemistry
• immunoprecipitation

生物化学的／生理学的作用

Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

調製ノート

バイオリアクタ-で増殖させたハイブリド-マ細胞の培養上清から精製しています。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable