SAE0044

コリンオキシダーゼ Arthrobacter sp.(アルスロバクター属) 由来

recombinant, expressed in E. coli ≥12 units/mg protein

• 別名: コリン:酸素 1-オキシドレダクターゼ
• Enzyme Commission number: 1.1.3.17 (BRENDA, IUBMB)
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: bacterial (arthrobacter sp.)
• 品質水準: 200
• リコンビナント: expressed in E. coli ≥12 units/mg protein
• フォーム: powder
• 比活性: ≥12 units/mg protein
• テクニック: activity assay: suitable
• UniProtアクセッション番号: A0A0Q9NRQ3; A0A0Q9PH20
• 保管温度: −20°C

詳細

詳細

Research area: Cell Signaling

Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the glucose–methanol–choline (GMC). The codA gene encodes the choline oxidase enzyme.

アプリケーション

Choline oxidase from Arthrobacter sp. has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection. It has been used for fluorescent detection of choline released during autotaxin (ATX) activity in conditioned media and plasma.

生物化学的／生理学的作用

Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the GMC-oxidoreductase family. The enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde, in two sequential FAD-dependent reaction steps. The enzyme is useful for enzymatic determination of phosphatidylcholine by coupling with phospholipase D or determination of sphingomyelin by coupling with sphingomyelinase and for cholinesterase activity assays.Choline oxidase (COD) mediating glycine betaine biosynthetic pathway plays a vital role in creating stress resistant transgenic plants.

単位の定義

One unit will form 1 μmole of H₂O₂ with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H₂O₂ are produced for every μmole of choline.

調製ノート

It is recommended to reconstitute choline oxidase in phosphate buffer at pH 7.5 supplemented with 10mM EDTA. Reconstituted material can be stored at 2-8 °C for at least 2 weeks.

保管および安定性

Tightly closed.Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable