T9784

トリシン

BioXtra, pH 4.0-6.0 (1 M in H₂O), ≥99% (titration)

• 別名: N-[トリス(ヒドロキシメチル)メチル]グリシン
• 化学式: (HOCH₂)₃CNHCH₂CO₂H
• CAS番号: 5704-04-1
• 分子量: 179.17
• Beilstein: 1937804
• EC Number: 227-193-6
• MDL番号: MFCD00004277
• UNSPSCコード: 12161700
• PubChem Substance ID: 24900603
• NACRES: NA.25

特性

• 製品種目: BioXtra
• 品質水準: 400
• アッセイ: ≥99% (titration)
• フォーム: powder
• 不純物: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• 強熱残分: ≤0.1%
• pH: 4.0-6.0 (1 M in H₂O)
• 有効pH範囲: 7.4-8.8
• pKa (25 °C): 8.1
• 溶解性: H₂O: 1 M, clear, colorless
• 微量陰イオン: chloride (Cl^-): ≤0.1%; sulfate (SO₄^2-): ≤0.05%
• 微量陽イオン: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• Absorption: ≤0.05 at 280 in H₂O at 1 M; ≤0.1 at 260 in H₂O at 1 M
• アプリケーション: diagnostic assay manufacturing
• SMILES記法: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N

詳細

詳細

The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.

アプリケーション

低分子量ペプチド分離用のバッファー成分です。

Tricine has been used:

• in a polymerase chain reaction (PCR) buffer used in the single-cell collection
• as a component of lactotroph feeding medium for culturing lactotrophs
• to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)