U211140

DMT-2'O-メチル-rU ホスホロアミダイト

configured for MerMade

• 別名: 5′-O-[ビス(4-メトキシフェニル)フェニルメチル]-2′-O-メチル-ウリジン, 3′-[2-シアノエチル N,N-ビス(1-メチルエチル)ホスホロアミダイト], DMT-2'-O-メチル-rU アミダイト
• 実験式（ヒル表記法）: C₄₀H₄₉N₄O₉P
• CAS番号: 110764-79-9
• 分子量: 760.81
• MDL番号: MFCD00792626
• UNSPSCコード: 41116105
• NACRES: NA.51

特性

• 由来生物: non-animal source (no BSE/TSE risk)
• 品質水準: 300
• 製品種目: Proligo Reagents
• アッセイ: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• フォーム: powder
• 分子量: 760.81 g/mol
• 不純物: ≤0.3% mU2 (reversed phase HPLC, Hydrolysate); ≤0.3% mU3 (reversed phase HPLC, DMT-rme); ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤0.5% single Impurity (redox titration); ≤1.0% mU1 (reversed phase HPLC, DMT-rUme-DMT); ≤3% residual Solvent content
• 色: white to off-white
• 適合性: conforms to structure for H-NMR; conforms to structure for LC-MS; configured for MerMade
• 保管温度: 2-8°C
• SMILES記法: CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1N5C=CC(=O)NC5=O
• InChI: 1S/C40H49N4O9P/c1-27(2)44(28(3)4)54(51-25-11-23-41)53-36-34(52-38(37(36)49-7)43-24-22-35(45)42-39(43)46)26-50-40(29-12-9-8-10-13-29,30-14-18-32(47-5)19-15-30)31-16-20-33(48-6)21-17-31/h8-10,12-22,24,27-28,34,36-38H,11,25-26H2,1-7H3,(H,42,45,46)/t34-,36-,37-,38-,54?/m1/s1
• InChI Key: UVUOJOLPNDCIHL-XKZJCBTISA-N

詳細

詳細

DMT-2′O-Methyl-rU Phosphoramidite belongs to the class of 2′O-Methyl RNA Phosphoramidites. Its key features include:

• High yield of crude oligonucleotides Compatible with DNA synthesis
• Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides
• Recommended deprotection conditions are 8 hours at 55 deg C using concentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 deg C
• Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation
• Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6minutes compared to 90 seconds for DNA monomers)
• 2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry

アプリケーション

2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rU Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of:

• Diagnostic probes
• Aptamer and ribozyme development
• Mixed 2′O-Methyl-RNA/DNA antisense molecules

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable