L040050

Cap A

configured for (ÄKTA^® and OligoPilot^®)

• UNSPSC 코드: 12352200
• NACRES: NA.21

속성

• 유형: for DNA synthesis
• Quality Level: 200
• 제품 라인: Proligo Reagents
• 형태: liquid
• 저장 조건: dry at room temperature
• 불순물: ≤100 ppm water content (Karl Fischer)
• 색상: clear
• 적합성: conforms to structure (GC, Formulation)
• 호환성: configured for (ÄKTA^® and OligoPilot^®)
• 저장 온도: room temp

설명

애플리케이션

Cap A solution is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

제조 메모

Acetonitrile / N-Methylimidazole 8/2 (v/v)

기타 정보

Cap A solution, containing acetonitrile (ACN) and N-methylimidazole (NMI), is mixed in situ with Cap B1 and Cap B2 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.

법적 정보

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

안전성 정보

• 픽토그램: GHS02,GHS06,GHS08,GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H225,H302 + H332,H311,H314,H361d
• 예방조치 성명서: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2 - Skin Corr. 1B
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point (°F): 35.6 °F
• Flash Point (°C): 2 °C