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Merck
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주요 문서

05-902R

Sigma-Aldrich

Anti-HA Tag Antibody, clone 114-2C-7, rabbit monoclonal

clone 114-2C-7, Upstate®, from rabbit

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.43

생물학적 소스

rabbit

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

114-2C-7, monoclonal

종 반응성(상동성에 의해 예측)

all

제조업체/상표

Upstate®

기술

western blot: suitable

동형

IgG

배송 상태

wet ice

타겟 번역 후 변형

unmodified

일반 설명

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. 05-902R is a recombinant version of the rabbit monoclonal Cat no. 05-902.

특이성

The antibody recognizes recombinant proteins containing the HA epitope tag.

면역원

Synthetic peptide containing the sequence ...CYPYDVPDY... derived from the influenza virus hemaglutinin (HA) protein.

애플리케이션

Anti-HA Tag Antibody, clone 114-2C-7 detects level of HA Tag & has been published & validated for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

품질

Routinely evaluated by Western Blot HA-tagged WNT-1 transfected HEK293 cells.

물리적 형태

Format: Purified
Protein A purified
Protein A purified rabbit immunoglobulin in buffer containing 60mM Tris, 90 mM NaCl, 0.03% NaN3, 60mM glycine, pH 7.5, and 40% glycerol.

저장 및 안정성

Maintain for 1 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Manuel Osorio-Valeriano et al.
Molecular cell, 81(19), 3992-4007 (2021-09-26)
ParB-like CTPases mediate the segregation of bacterial chromosomes and low-copy number plasmids. They act as DNA-sliding clamps that are loaded at parS motifs in the centromere of target DNA molecules and spread laterally to form large nucleoprotein complexes serving as
Anna Nordin et al.
Genome biology, 24(1), 185-185 (2023-08-11)
Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is an increasingly popular technique to map genome-wide binding profiles of histone modifications, transcription factors, and co-factors. The ENCODE project and others have compiled blacklists for ChIP-seq which have been widely adopted:
Dario Zimmerli et al.
eLife, 9 (2020-08-19)
BCL9 and PYGO are β-catenin cofactors that enhance the transcription of Wnt target genes. They have been proposed as therapeutic targets to diminish Wnt signaling output in intestinal malignancies. Here we find that, in colorectal cancer cells and in developing
Rongkun Li et al.
Molecular cancer, 18(1), 18-18 (2019-02-02)
Lysyl oxidase-like 4 (LOXL4) has been found to be dysregulated in several human malignancies, including hepatocellular carcinoma (HCC). However, the role of LOXL4 in HCC progression remains largely unclear. In this study, we investigated the clinical significance and biological involvement
Jing Xu et al.
PloS one, 7(12), e52799-e52799 (2012-12-20)
Conjugation in Tetrahymena thermophila involves a developmental program consisting of three prezygotic nuclear divisions, pronuclear exchange and fusion, and postzygotic and exconjugant stages. The conjugation junction structure appears during the initiation of conjugation development, and disappears during the exconjugant stage.

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