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Merck
모든 사진(1)

주요 문서

AB125

Sigma-Aldrich

Anti-Serotonin Antibody

serum, Chemicon®

동의어(들):

5-HT

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
클론:
polyclonal
application:
IHC
종 반응성:
rat
기술:
immunohistochemistry: suitable
citations:
9

생물학적 소스

rabbit

Quality Level

항체 형태

serum

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

rat

제조업체/상표

Chemicon®

기술

immunohistochemistry: suitable

배송 상태

dry ice

타겟 번역 후 변형

unmodified

특이성

Serotonin (5-Hydroxytryptamine)

The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:



Compound Cross-reactivity

5-Hydroxytryptamine-G-PL 1

5-Methoxytryptamine-G-PL 1/25

Tryptamine-G-PL 1/60

5-Hydroxytryptophan-G-PL 1/175

5-Hydroxytryptamine 1/1150

Tryptophan-G-PL 1/3400

5-Methoxytryptophan-G-PL 1/19800



The antisera was also tested for specificity using the free-floating PAP technique on rat

raphe nuclei.



Abbreviations:



(G) Glutaraldehyde

(=) Non-reduced conjugate

(PL) Poly-lysine

면역원

5-Hydroxytryptamine-glutaraldehyde-Poly-lysine

애플리케이션

Anti-Serotonin Antibody detects level of Serotonin & has been published & validated for use in IH.
Immunohistochemistry: 1:1,000-1:2,500 by ABC or enhanced detection. Glutaraldehyde fixation is required. Antibody will not react with non-glutaraldehyde treated tissues. Blocking should be with serum or BSA containing 0.1-0.2% triton X-100; remove all blocking an detergents during primary antibody incubation. Dilute only in PBS or TBS for best results. For fluorescent detection antibody dilution must be reduced, but it is not recommended because of glutaraldehyde autofluorescence.



Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

물리적 형태

Rabbit antiserum. Liquid with 0.05% sodium azide.

저장 및 안정성

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Shih-Chang Hsueh et al.
International journal of molecular sciences, 19(2) (2018-02-09)
Parkinson's disease (PD) is typically characterized by impairment of motor function. Gait disturbances similar to those observed in patients with PD can be observed in animals after injection of neurotoxin 6-hydroxydopamine (6-OHDA) to induce unilateral nigrostriatal dopamine depletion. Exercise has
Jun Lu et al.
The Journal of comparative neurology, 508(4), 648-662 (2008-04-03)
Classical anesthetics of the gamma-aminobutyric acid type A receptor (GABA(A))-enhancing class (e.g., pentobarbital, chloral hydrate, muscimol, and ethanol) produce analgesia and unconsciousness (sedation). Dissociative anesthetics that antagonize the N-methyl-D-aspartate (NMDA) receptor (e.g., ketamine, MK-801, dextromethorphan, and phencyclidine) produce analgesia but
Katia Carneiro et al.
BMC developmental biology, 11, 29-29 (2011-05-24)
Consistent asymmetry of the left-right (LR) axis is a crucial aspect of vertebrate embryogenesis. Asymmetric gene expression of the TGFβ superfamily member Nodal related 1 (Nr1) in the left lateral mesoderm plate is a highly conserved step regulating the situs
Sujan Gautam et al.
iScience, 27(1), 108616-108616 (2024-01-05)
Studies across a diverse group of metazoan embryos indicate that Wnt signaling often activates the transcription factor Sp5, forming a signaling 'cassette' that plays critical roles in many developmental processes. This study explores the role of Wnt/Sp5 signaling during the
Robert A Kozol et al.
eLife, 12 (2023-07-27)
The vertebrate brain is highly conserved topologically, but less is known about neuroanatomical variation between individual brain regions. Neuroanatomical variation at the regional level is hypothesized to provide functional expansion, building upon ancestral anatomy needed for basic functions. Classically, animal

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