CC052

Human Collagen Type II

liquid, 1 mg/mL, suitable for cell culture, used for gel formation

• 동의어(들): Collagen Type II, Human Collagen
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

속성

• product name: Human Collagen Type II,
• 생물학적 소스: human
• Quality Level: 100
• 분석: 90% (Human collagen type II)
• 형태: liquid
• 제조업체/상표: Chemicon^®
• 농도: 1 mg/mL
• 기술: cell culture | mammalian: suitable
• 불순물: <0.5% Non-collagen proteins; <1% Human collagen type IV; <1% Human collagen type V; <10% Human collagen type I; <10% Human collagen type III
• 입력: sample type pancreatic stem cell(s); sample type: human embryonic stem cell(s); sample type epithelial cells; sample type induced pluripotent stem cell(s); sample type mesenchymal stem cell(s); sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• solubility: water: soluble at 20 °C
• NCBI 수납 번호: NM_001844.4; NM_033150.2
• UniProt 수납 번호: P02458
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... COL2A1(1280)

설명

일반 설명

Type II collagen, a fibrillar collagen, is the main component of hyaline cartilage and constitutes 95% of the collagens and approximately 60% of cartilage’s dry weight. It is a homotrimer protein encoded by the gene COL2A1, mapped to human chromosome 12q13. Type two Collagen is also found in the nucleous pulposo of the intervertebral disc and the retina, sclera, and lens of the eye. Type IIA, is localized mainly to the vitreous body of the eye. Type IIB, is preferentially produced in adult cartilage tissue.  Collagen type II was purified by using differential salt precipitation, alcohol precipitation and DEAE chromatography. Multiple purification steps provide final preparations of collagen types in the native triple-helical form, free of any non-collagenous protein. Contamination of other collagen types in a standard preparation is lower than five percent.

애플리케이션

Human Collagen Type II has been used:

• as an extracellular matrix (ECM) protein in microarray analysis, to coat on nitrocellulose film slides for probing and detection experiments
• to study the role of Col2a1a in the neural crest during early eye development in zebrafish model of Stickler syndrome
• as a calibration standard in the quantification of collagen II levels in scaffolds using an enzyme-linked immunosorbent assay (ELISA)-based assay

생화학적/생리학적 작용

Type II collagen is commonly co-assembled with collagen XI, forming a complex that is covalently crosslinked to collagen IX and interacts with small leucine-rich proteoglycans. Collagen 2 imparts stability and strength to the tissue and maintains integrity and resilience under stress. Cleavage of Type II collagen occurs mainly through the action of matrix metalloproteinase collagenases, leading to the generation of well-known biomarkers such as the C-terminal telopeptide of Type II collagen and C2C. Mutations in type II collagen cause chondrodysplasia, leading to premature osteoarthritis.

물리적 형태

Purified protein. Liquid in 0.5M acetic acid. No preservative.

저장 및 안정성

Aliquot and freeze. Maintain at -20°C up to 12 months. Avoid repeated freeze/thaw cycles.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

안전성 정보

• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable