C9301

Chicken Collagen Type II

from chicken sternal cartilage, powder, suitable for cell culture

• CAS Number: 9007-34-5
• EC Number: 232-697-4
• MDL number: MFCD00130825
• UNSPSC 코드: 12352202
• NACRES: NA.75

속성

• product name: Collagen from chicken sternal cartilage, Type II (Miller), powder, BioReagent, suitable for cell culture
• 생물학적 소스: chicken (Sternal cartilage)
• Quality Level: 300
• 유형: Type II (Miller)
• 제품 라인: BioReagent
• 형태: powder
• 포장: glass bottle of 100 mg; poly bottle of 25 mg; glass bottle of 5 mg
• 농도: 60-80% (biuret)
• 기술: cell culture | mammalian: suitable
• 표면 범위: 6‑10 μg/cm²
• solubility: acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
• UniProt 수납 번호: P02460
• 결합 특이성: Peptide Source: Fibrinogen
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 유전자 정보: chicken ... COL2A1(395069)

설명

일반 설명

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

애플리케이션

Collagen from chicken sternal cartilage has been used:

• in enzyme–linked immunosorbent assay (ELISA).
• chondrocyte-mediated tissue production in vitro.
• induction, treatment and assessment of collagenα induced arthritis (CIA).
• cell proliferation assay.
• as a coating for cell culture surfaces.

생화학적/생리학적 작용

Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.

특징 및 장점

This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.

기타 정보

Major component of articular cartilage.

제조 메모

Prepared by a modification of the method of Trentham, D.E., et al.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)