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Merck
모든 사진(1)

주요 문서

MAB1083

Sigma-Aldrich

Anti-GFP Antibody, clone 3F8.2

clone 3F8.2, from mouse

동의어(들):

Green Fluorescent protein

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

3F8.2, monoclonal

종 반응성

vertebrates

기술

western blot: suitable

동형

IgG1κ

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

일반 설명

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

특이성

Reacts with most common vertebrate proteins containing the GFP tag.
The antibody reacts with the 28 kDa GFP protein.

면역원

Epitope: Full Length
Full length GFP protein.

애플리케이션

Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Epitope Tags
Western Blot Analysis:
0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).

품질

Routinely evaluated by Western Blot on GFP recombinant protein.

표적 설명

28 kDa

물리적 형태

Format: Purified
Protein G Purified
Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide.

저장 및 안정성

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
Western Blot: GFP recombinant protein

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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