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Merck
모든 사진(1)

주요 문서

MSIPS4W10

Millipore

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, sterile

동의어(들):

Hydrophobic 96-Well Plate, PVDF 96-Well Plate

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About This Item

UNSPSC 코드:
41104923
eCl@ss:
32039006
NACRES:
NB.22
material:
PVDF membrane (hydrophobic)
acrylic
flat bottom wells
공극 크기:
0.45 μm pore size
무균:
sterile
결합 유형:
high binding surface

material

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

Quality Level

설명

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

무균

sterile

제품 라인

MultiScreen®

특징

hydrophobic
lid

제조업체/상표

MultiScreen®

파라미터

50-250 μL sample volume (per well)

기술

ELISPOT: suitable

여과 면적

0.28 cm2

플레이트 크기

96 wells

웰 최대 용적

300 μL

작업 용적

50-250 μL

Matrix

Immobilon®-P

공극 크기

0.45 μm pore size

결합 유형

high binding surface

배송 상태

ambient

일반 설명

The polyvinylidene fluoride (PVDF) membrane plates are recommended, over a mixed cellulose ester format, due to slightly improved binding of capture antibody and superior performance in spot detection, particularly for fluorescent applications.

애플리케이션

MultiScreen® HTS IP Filter Plate, 0.45 μm, white, sterile has been used in enzyme-linked immunospot (ELISpot) assay using:
  • dsDNA-producing plasma cells
  • memory B cells and bone marrow localized long-lived plasma cells
  • draining lymph node and spleen cells

법적 정보

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Katrin Klocke et al.
Immunology, 152(1), 125-137 (2017-05-13)
Cytotoxic T-lymphocyte antigen 4 (CTLA-4) -mediated regulation of already tolerized autoreactive T cells is critical for understanding autoimmune responses. Although defects in CTLA-4 contribute to abnormal FOXP3+ regulatory T (Treg) cell function in rheumatoid arthritis, its role in autoreactive T
Mubeen M Mosaheb et al.
Nature communications, 11(1), 524-524 (2020-01-29)
Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of
Jing Zhu et al.
Journal of immunology (Baltimore, Md. : 1950), 204(5), 1091-1100 (2020-01-29)
Almost a decade has passed since the approval of belimumab, an mAb directed against B lymphocyte stimulation and the first targeted therapy approved for systemic lupus erythematous (SLE) in over 50 y. Although well tolerated, the efficacy of belimumab remains
Leah E Cole et al.
Vaccines, 8(4) (2020-12-06)
While both whole-cell (wP) and acellular pertussis (aP) vaccines have been highly effective at reducing the global pertussis disease burden, there are concerns that compared to wP vaccination, the immune responses to aP vaccination may wane more rapidly. To gain

문서

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

프로토콜

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

관련 콘텐츠

This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.

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