추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
monoclonal
양식
lyophilized
포장
pkg of 100 μg
제조업체/상표
Roche
동형
IgG1
저장 온도
2-8°C
일반 설명
Anti-His6 is a monoclonal antibody to His6-tagged proteins. Anti-His6 specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the location of the histidine-tag in the protein sequence.
특이성
Anti-His6 specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the histidine-tag in the protein sequence. In addition, Anti-His6 preferentially recognizes the C-terminal His6-epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.
애플리케이션
Anti-His6 is used for the detection of His6-tagged proteins in:
- Immunoblotting (e.g., dot blots and western blots)
- Immunoprecipitation
- Immunoassays (ELISA)
- Immunocytochemistry
- Immunofluorescence
제조 메모
Stabilizers: BSA
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
- ELISA: for detection 0.1 μg/ml; for coating 1 to 5 μg/ml
- Immunoprecipitation: 0.5 to 5 μg/ml
- Western blot: 0.2 to 0.5 μg/ml
재구성
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
기타 정보
For life science research only. Not for use in diagnostic procedures.
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
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