70796-M

His•Tag^® Monoclonal Antibody

Novagen^®

• 동의어(들): Anti-His antibody
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 항체 형태: purified antibody
• Quality Level: 100
• 제조업체/상표: Novagen^®
• 저장 조건: OK to freeze
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Sensitive, specific detection of His•Tag fusion proteins

The HIS TAG^® Monoclonal Antibody is a mouse monoclonal antibody (IgG₁) directed against the HIS TAG^® sequence encoded by many of the NOVAGEN^® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (K_d = 5 × 10^-8 to 1 × 10^-9 M) enables sensitive, specific detection of HIS TAG^® fusion proteins at antibody concentrations of 0.1-0.2 g/ml. The HIS TAG^® Monoclonal Antibody binds to N-terminal, C-terminal, and internal HIS TAG^® sequences. This antibody also detects the recombinant marker bands in the Perfect Protein<TMSYMBOL></TMSYMBOL> and Trail Mix<TMSYMBOL></TMSYMBOL> Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-g package size provides enough purified antibody for 1000 mL of working solution.

* Not recommended for HRP-based detection of Trail Mix Western Markers.

경고

Toxicity: Standard Handling (A)

법적 정보

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable