SAB4200620
Monoclonal Anti-polyHistidine antibody produced in mouse
clone HIS-1, purified immunoglobulin
동의어(들):
Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag
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모든 사진(3)
About This Item
UNSPSC 코드:
12352203
NACRES:
NA.41
추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
HIS-1, monoclonal
포장
antibody small pack of 25 μL
농도
~1.0 mg/mL
기술
flow cytometry: 3-6 μg/test
immunoblotting: 0.25-0.5 μg/mL
immunofluorescence: 4-8 μg/mL
동형
IgG2a
저장 온도
−20°C
타겟 번역 후 변형
unmodified
일반 설명
Monoclonal Anti-polyHistidine (mouse IgG2a isotype) is derived from the HIS-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant His-tagged fusion protein. The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor. Monoclonal anti-polyHistidine recognizes native as well as denatured, reduced forms of synthetic polyHistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET, and pTrc expression vectors. The antibody preferentially recognizes N-terminal tagged fusion proteins.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
저장 및 안정성
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
기타 정보
In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration. Use of sensitive film is recommended.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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