COLLH-RO

Collagenase H

from Clostridium histolyticum

• 효소 위원회 번호: 3.4.24.3 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: Clostridium histolyticum
• Quality Level: 100
• 무균: non-sterile
• 형태: lyophilized
• collagenase activity: 0.15 U/mg lyophilizate
• 포장: pkg of 100 mg (11074032001); pkg of 2.5 g (11087789001); pkg of 500 mg (11074059001)
• 제조업체/상표: Roche
• 농도: ~1 mg/mL (Isolation of rat hepatocytes); ~2 mg/mL (Isolation of adipocytes)
• 기술: tissue processing: suitable
• 색상: brown
• 최적 pH: 6.0-8.0
• solubility: water: soluble
• NCBI 수납 번호: WP_138210331
• UniProt 수납 번호: Q46085
• 응용 분야: life science and biopharma
• 외래 활성: Clostripain 39.798 U/mg; Proteases 14.014 U/mg (Azocoll, based on lyoph); Trypsin 1.668 U/mg (with BAEE)
• 저장 온도: 2-8°C

설명

일반 설명

Collagenase H is an enzyme mixture prepared from Clostridium histolyticum cultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization.

특이성

Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases also degrade other proteins.

애플리케이션

Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Collagenase H is well suited for the isolation of hepatocytes from rat liver by the collagenase perfusion method. This enzyme preparation is also suitable for the preparation of other types of cells such as endothelial cells from large vessels, and for the isolation of adipocytes from epididymal fat pads of rats.

물리적 형태

Lyophilizate, nonsterile

제조 메모

Enzyme activity:
Collagenase activity: >0.15U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate).
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase H has a balanced ratio of enzyme activities, and is function tested for the isolation of rat hepatocytes (perfusion method).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Activator: Ca²⁺
Working concentration: 0.5 to 2.5mg/ml,
Approximately 1 mg/ml for the isolation of rat hepatocytes and 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution should be stored at -15 to -25°C for short term storage, -60°C or as described below for long-term storage.

재구성

Reconstitution in any balanced salt solution (e.g., HBSS)

저장 및 안정성

Keep container tightly closed in a dry and well-ventilated place.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS07,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H315 - H319 - H334 - H335
• 예방조치 성명서: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash