LEU-RO

Leupeptin

powder, from synthetic, =96.5%

• 동의어(들): Leupeptin hemisulfate salt, Acetyl-Leu-Leu-Arg-al, N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate salt
• Linear Formula: C₂₀H₃₈N₆O₄ · 1/2H₂SO₄
• CAS Number: 103476-89-7
• Molecular Weight: 475.59
• MDL number: MFCD00037012
• UNSPSC 코드: 12352202
• PubChem Substance ID: 329817679

속성

• 생물학적 소스: synthetic
• Quality Level: 100
• 설명: Ac-Leu-Leu-argininal x 1/2 H₂SO₄, synthetic
• 분석: 96.5%
• 양식: powder
• 분자량: M_r 475.6
• 포장: pkg of 100 mg (11529048001); pkg of 25 mg (11017128001); pkg of 5 mg (11017101001); pkg of 50 mg (11034626001)
• 제조업체/상표: Roche
• solubility: H₂O: 50 mg/mL
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• SMILES string: OS(O)(=O)=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O
• InChI: 1S/2C20H38N6O4.H2O4S/c2*1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;1-5(2,3)4/h2*11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1
• InChI key: CIPMKIHUGVGQTG-VFFZMTJFSA-N

설명

특이성

Leupeptin inhibits serine and thiol proteases such as trypsin, plasmin, proteinase K, kallikrein, papain, thrombin, and cathepsin A and B.
Not affected are α-, β-, γ- and δ- chymotrypsin, pepsin, cathepsin D, elastase, renin, and thermolysin.

애플리케이션

Leupeptin as well as other protease inhibitors like antipain, chymostatin, pepstatin, and phosphoramidon are useful for the protection of proteins during their isolation from tissues or membranes. Leupeptin can be removed from the reaction by dialysis.
Note: To check other protease inhibitors, try our Protease Inhibitor Set including Antipain Dihydrochloride, Aprotinin, Bestatin, Chymostatin, E-64, EDTA-Na₂, Leupeptin, Pefabloc SC, Pepstatin, and Phosphoramidon.

생화학적/생리학적 작용

Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)

특징 및 장점

Contents
Synthetic, white powder

품질

Function test: Performance controlled with trypsin.

제조 메모

Working concentration: 0.5 to 5 μg/ml (1 to 20 μM)

Comparison of working concentrations of pefabloc with leupeptin and PMSF is in files.

Working solution: Solvent is recommended in distilled water.
Highly soluble in water (1 mg/ml), methanol, ethanol, acetic acid, dimethyl formamide and dimethyl sulfoxide.
Poorly soluble in acetone, chloroform, ethyl ether and n-hexane.

Storage conditions (working solution): -15 to -25 °C
In aqueous solution leupeptin is stable for 1 month at 2 to 8 °C or for at least 6 months at - 15 to -25 °C, stored under nitrogen. For best results, freeze the dissolved inhibitor in aliquots and avoid repeated thawing.

재구성

Highly soluble in water (1 mg/ml). Stable for at least one week at 2 to 8 °C and 6 months frozen in aliquots at -15 to -25 °C.

분석 메모

Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined using three main peaks. Majority of contaminating peptide is racemized leupeptin.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302 + H312 + H332
• 예방조치 성명서: P261 - P264 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable