67309

Kovac′s reagent for indoles

suitable for microbiology

• 동의어(들): 4-(Dimethylamino)benzaldehyde solution
• 실험식(Hill 표기법): C₉H₁₁NO
• CAS Number: 100-10-7
• Molecular Weight: 149.19
• Beilstein: 4132845
• MDL number: MFCD00003381
• UNSPSC 코드: 41171621
• PubChem Substance ID: 329760541
• NACRES: NA.85

속성

• Agency: according to ISO 16654:2001
• Quality Level: 200
• 제품 라인: BioChemika
• 유통기한: limited shelf life, expiry date on the label
• 구성: 4-(dimethlyamino)benzaldehyde, 50 g/L ; hydrochloric acid, 240 g/L ; isoamylic alcohol, 710 g/L
• 기술: microbe id | specific enzyme detection: suitable
• 응용 분야: agriculture; clinical testing; environmental; food and beverages; microbiology
• 저장 온도: 2-8°C
• 적합성: Escherichia coli; coliforms
• SMILES string: [H]C(=O)c1ccc(cc1)N(C)C
• InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
• InChI key: BGNGWHSBYQYVRX-UHFFFAOYSA-N

설명

일반 설명

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

애플리케이션

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.

Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

안전성 정보

• 픽토그램: GHS02,GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H226,H290,H314,H317,H332,H335
• 예방조치 성명서: P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point (°F): 109.4 °F
• Flash Point (°C): 43 °C
• 개인 보호 장비: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter