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Merck
모든 사진(1)

주요 문서

07373

Sigma-Aldrich

Phalloidin–Atto 740

BioReagent, suitable for fluorescence

동의어(들):

Atto 740–Phalloidin

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About This Item

UNSPSC 코드:
12352108
NACRES:
NA.32

제품 라인

BioReagent

Quality Level

분석

≥90.0% (HPLC)

양식

powder

제조업체/상표

ATTO-TEC GmbH

투과

254 nm
740 nm

형광

λex 750 nm; λem 764 nm in 0.1 M phosphate pH 7.0

UV 흡수

λ: 739 -745 nm Amax

적합성

suitable for fluorescence

저장 온도

−20°C

일반 설명

Atto 740 belongs to a new generation of fluorescent labels for the near infrared spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the dye are strong absorption and good fluorescence as well as excellent thermal and photo-stability. Atto 740 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
Atto 740 is a pH sensitive product. While practically stable up to pH 7.4 (PBS-buffer), it slowly degrades at higher pH. If exposed to higher pH for coupling purposes, we recommend reducing the pH immediately after completion of the reaction.
Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10-8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-d-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.

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포장

Bottomless glass bottle. Contents are inside inserted fused cone.

법적 정보

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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