71736

Sodium dodecyl sulfate solution

BioUltra, for molecular biology, 10% in H₂O

• 동의어(들): Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate solution
• Linear Formula: CH₃(CH₂)₁₁OSO₃Na
• CAS Number: 151-21-3
• Molecular Weight: 288.38
• Beilstein: 3599286
• MDL number: MFCD00036175
• UNSPSC 코드: 12161902
• eCl@ss: 39093306
• PubChem Substance ID: 57652355
• NACRES: NA.26

속성

• Grade: for molecular biology
• 설명: anionic
• 제품 라인: BioUltra
• 형태: liquid
• 분자량: 288.38 g/mol
• 농도: 10% in H₂O
• 기술: HPLC: suitable; protein quantification: suitable
• 불순물: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 5.0-6.5
• density: 1.01 g/mL at 20 °C
• 음이온 미량물: chloride (Cl^-): ≤50 mg/kg; phosphate (PO₄^3-): ≤1 mg/kg
• 양이온 미량물: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• UV 흡수: λ: 260 nm A_max: 0.3; λ: 280 nm A_max: 0.2
• SMILES string: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• InChI key: DBMJMQXJHONAFJ-UHFFFAOYSA-M

설명

애플리케이션

Sodium dodecyl sulfate solution has been used:

• in the preparation of 2x extraction buffer
• as a component in elution buffer for immunoprecipitation
• in SDS–polyacrylamide gel electrophoresis (PAGE) analysis

생화학적/생리학적 작용

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids. It is also considered as a permeability enhancer in oral formulations.

기타 정보

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H315,H319
• 예방조치 성명서: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter