추천 제품
생물학적 소스
mouse
Quality Level
결합
alkaline phosphatase conjugate
항체 형태
purified immunoglobulin
항체 생산 유형
secondary antibodies
클론
RG-96, monoclonal
양식
buffered aqueous glycerol solution
종 반응성
rabbit
반응하면 안 됨
goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
기술
direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells
동형
IgG1
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
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일반 설명
Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
애플리케이션
Monoclonal Anti-Rabbit IgG (γ-chain specific)Alkaline Phosphatase antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
생화학적/생리학적 작용
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
물리적 형태
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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시험 성적서(COA)
Lot/Batch Number
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