추천 제품
제품명
Amphiregulin human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
생물학적 소스
human
Quality Level
재조합
expressed in E. coli
분석
≥97% (SDS-PAGE)
양식
lyophilized powder
효능
10-100 ng/mL ED50/EC50
분자량
~11 kDa
포장
pkg of 1 mg
저장 조건
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
기술
cell culture | mammalian: suitable
불순물
endotoxin, tested
UniProt 수납 번호
저장 온도
−20°C
유전자 정보
human ... AREG(374)
생화학적/생리학적 작용
Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.
성분
Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.
주의사항
This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.
물리적 형태
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.
제조 메모
It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.
분석 메모
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
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The Journal of experimental medicine, 210(13), 2951-2965 (2013-11-20)
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General and comparative endocrinology, 180, 39-47 (2012-11-28)
Little is known about the expression and regulation of epiregulin (EREG) and amphiregulin (AREG) in ovarian follicles of large monoovulatory animal species. To characterize the gonadotropin-dependent regulation of EREG and AREG mRNAs in equine follicles prior to ovulation, extracts were
Mathew C Casimiro et al.
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