A7607

Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse

~1.0 mg/mL, clone CGA7, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD00162107
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: CGA7, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~42 kDa
• 종 반응성: rat, chicken, mouse, human, monkey
• 농도: ~1.0 mg/mL
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 5-10 μg/mL using chicken gizzard total extract
• 동형: IgG2b
• UniProt 수납 번호: P62736
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: chicken ... ACTA2(423787) ; human ... ACTA2(59) ; mouse ... Acta2(11475) ; rat ... Acta2(81633)

설명

일반 설명

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. Its expression is related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha actins (skeletal, cardiac, smooth), one beta actin (beta-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle).
Monoclonal Anti-Actin, Smooth Muscle, reacts with human, monkey, chicken, rat, and mouse smooth muscle actin.

Monoclonal Anti-Actin, Smooth Muscle (mouse IgG2b isotype) is derived from the hybridoma CGA7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with partially purified chicken gizzard actin. α-actin 2 (ACTA2) codes for a smooth muscle specific α-actin.

The antibody specifically recognizes α and γ smooth muscle actin.

면역원

partially purified chicken gizzard actin.

애플리케이션

Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• immunohistochemistry

생화학적/생리학적 작용

Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Mutations in alpha-actin 2 (ACTA2) results in thoracic aortic aneurysm, Moyamoya disease, coronary artery disease, stroke and multisystemic smooth muscle dysfunction syndrome. It plays a functional role in the smooth muscle of non-vascular tissues.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)