A9770

Aldehyde Dehydrogenase, potassium-activated from yeast

lyophilized powder, ≥10 units/mg protein

• 동의어(들): ALDH, CoA-independent aldehyde dehydrogenase, m-methylbenzaldehyde dehydrogenase, Aldehyde:NAD[P]⁺ oxidoreductase
• CAS Number: 9028-88-0
• 효소 위원회 번호: 1.2.1.5 (BRENDA, IUBMB)
• EC Number: 232-846-3
• MDL number: MFCD00081306
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: yeast
• Quality Level: 200
• 형태: lyophilized powder
• 특이 활성도: ≥10 units/mg protein
• 저장 조건: (Tightly closed. Dry)
• 색상: white
• UniProt 수납 번호: A0A3G3NDH9; S5RCH3; S5RK20; S5S176
• 외래 활성: NADH oxidase ≤0.01%; NADPH oxidase ≤0.01%; alcohol dehydrogenase ≤0.01%; lactic dehydrogenase ≤0.01%
• 저장 온도: 2-8°C

설명

일반 설명

Aldehyde dehydrogenase, from yeast, is used to catalyze the oxidation of a wide range of substrates, such as acetaldehyde, formaldehyde, propionaldehyde, n-butylaldehyde, isobutylaldehyde, n-valeraldehyde, caproaldehyde, benzaldehyde, glycoaldehyde, D-glyceraldehyde, malonic semialdehyde, and succinic aldehyde. Aldehyde dehydrogenase, from yeast has been used to study the production of ethanol and isobutanol. Aldehyde dehydrogenase, from Sigma, has been used along with alcohol dehydrogenase to measure ethanol production during the characterization of glycolytic metabolism and ion transport in Candida albicans.

The family of aldehyde dehydrogenase (ALDH) contains 19 genes in humans. It is localized in the nucleus, cytosol, mitochondria and endoplasmic reticulum of the cell.

Research area: Cell Signaling

애플리케이션

Aldehyde Dehydrogenase, potassium-activated from yeast has been used:

• for enzyme immobilization and to oxidize formaldehyde to formate
• to study the functional relation between hydrazone
• to measure ethanol production.

생화학적/생리학적 작용

Aldehyde dehydrogenase (ALDH) modulates the non-P450 aldehyde reduction enzyme system. It protects the cell from the effects of toxic aldehydes. Mutations in this gene leads to Sjogren‐Larsson syndrome, Larsson syndrome, type II hyperprolinemia and cancer. ALDH-2 lowers cardiac ischemia, which arises due to myocardial infarction or post cardiac surgery.Aldehyde Dehydrogenase (ALDH) participates in the functioning of regulatory T cells that are a part of the immune system. It is also involved in cellular detoxification, the amino acid metabolism and protects cells against ultraviolet (UV) rays-induced damage. ALDH plays a role in retinoic acid biosynthesis and signaling.

Aldehyde dehydrogenase is a tetramer and has several different isoforms. Aldehyde dehydrogenase is inhibited by propylurea, crotonaldehyde, n-propyl isocyanate, cyclohexyl isocyanate, 1-n-propyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea, and 1-methyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea. The enzyme tested in 0.01 M pyrophosphate buffer shows a sharp optimum around pH 9.3 with acetaldehyde as substrate.

단위 정의

One unit will oxidize 1 micromole of acetaldehyde to acetic acid per minute pH 8.0 at 25 deg C.

물리적 형태

Contains potassium phosphate salts

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)