C0378

Chloramphenicol

≥98% (HPLC)

• 동의어(들): D-(−)-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, D-(−)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol, D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]acetamide, Chloromycetin
• Linear Formula: Cl₂CHCONHCH(CH₂OH)CH(OH)C₆H₄NO₂
• CAS Number: 56-75-7
• Molecular Weight: 323.13
• Beilstein: 2225532
• EC Number: 200-287-4
• MDL number: MFCD00078159
• UNSPSC 코드: 51102831
• PubChem Substance ID: 24892250
• NACRES: NA.76

속성

• Quality Level: 200
• 분석: ≥98% (HPLC)
• 형태: powder or crystals
• pKa: 5.5
• mp: 149-153 °C (lit.)
• 항생제 활성 스펙트럼: Gram-negative bacteria; Gram-positive bacteria; mycobacteria; mycoplasma
• 동작 모드: protein synthesis | interferes
• 저장 온도: room temp
• SMILES string: OC[C@@H](NC(=O)C(Cl)Cl)[C@H](O)c1ccc(cc1)[N+]([O-])=O
• InChI: 1S/C11H12Cl2N2O5/c12-10(13)11(18)14-8(5-16)9(17)6-1-3-7(4-2-6)15(19)20/h1-4,8-10,16-17H,5H2,(H,14,18)/t8-,9-/m1/s1
• InChI key: WIIZWVCIJKGZOK-RKDXNWHRSA-N
• 유전자 정보: human ... CYP1A2(1544)

설명

일반 설명

Chemical structure: phenicole

애플리케이션

Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It is often used for bacterial selection in molecular biology applications at 10-20μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.
Chloramphenicol has been used as a bacteriostatic non potent antibiotic. It has been used for the selection/growth of positive bacterial cells.

생화학적/생리학적 작용

Mode of Action: Chloramphenicol inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome. It also inhibits mitochondrial and chloroplast protein synthesis and ribosomal formation of (p)ppGpp, de-pressing rRNA transcription.

Mode of Resistance: Use of chloramphenicol acetyltransferase will acetylate the product and inactivate it.

Antimicrobial Spectrum: This is a broad spectrum antibiotic against gram-positive and gram-negative bacteria, and is used mainly for ophthalmic and veterinary purposes.

주의사항

Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.

제조 메모

Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.

기타 정보

Keep container tightly closed in a dry and well-ventilated place.

안전성 정보

• 픽토그램: GHS08,GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H318,H351,H361fd
• 예방조치 성명서: P202 - P280 - P305 + P351 + P338 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2 - Eye Dam. 1 - Repr. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges