추천 제품
생물학적 소스
human blood (monocyte)
포장
pkg of 1,500,000 cells
형태학
(macrophages)
기술
cell culture | mammalian: suitable
배송 상태
dry ice
저장 온도
−196°C
일반 설명
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
세포주 기원
Monocyte
애플리케이션
Cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in M1 Macrophage Generation Media DXF and are available as non-activated, fully qualified M1- (GM-CSF) polarized cells. After 9-10 days of differentiation, the M1 macrophages are cryopreserved using Cryo-SFM. Each cryo vial contains more than 1.5 million viable cells after thawing.
경고
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
계대배양 정규 작업
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기타 정보
Recommended Plating Density: 100000 cells per cm2Tested Markers: CD80 positive, CD68 positiveNote: The plated cells do not proliferate. Recommended plating density for obtaining an adherent cell monolayer with 70-90% confluency.
면책조항
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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문서
Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.
프로토콜
Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.
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