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Merck
모든 사진(1)

주요 문서

CYTOCOX1

Sigma-Aldrich

Cytochrome c Oxidase Assay Kit

sufficient for 100 tests, soluble and membrane bound mitochondria

동의어(들):

Cytochrome c Oxidase Activity Assay Kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

Quality Level

사용

sufficient for 100 tests

배송 상태

dry ice

저장 온도

−20°C

유전자 정보

human ... COX1(4512)

애플리케이션

The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.

생화학적/생리학적 작용

Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.

특징 및 장점

  • Simple, optimized protocol - Obtain reproducible results without special training needs
  • Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
  • Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
  • May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
  • Use to analyze the intactness of mitochondrial membranes
  • Simple colorimetric measurement of solution

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS

  • C2506Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mgSDS

  • D4641n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mgSDS

픽토그램

Corrosion

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

이미 열람한 고객

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The Journal of biological chemistry, 284(12), 7553-7560 (2009-01-23)
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Nutritional neuroscience, 20(9), 538-546 (2016-06-23)
The activities of mitochondrial enzymes, which are essential for neural function, decline with age and in age-related disease. In particular, the activity of cytochrome c oxidase (COX/complex IV) decreases in patients with Alzheimer's disease (AD). COX, a mitochondrial inner membrane
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PloS one, 6(12), e28536-e28536 (2011-12-24)
Human primary myotubes are highly glycolytic when cultured in high glucose medium rendering it difficult to study mitochondrial dysfunction. Galactose is known to enhance mitochondrial metabolism and could be an excellent model to study mitochondrial dysfunction in human primary myotubes.

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