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Merck
모든 사진(2)

주요 문서

CS0720

Sigma-Aldrich

Citrate Synthase Assay Kit

1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

동의어(들):

Citrate Synthase Activity Test Kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.28

Quality Level

사용

 kit sufficient for 100 reactions (using a 1 ml cuvette)
 kit sufficient for 480 reactions (using 96 multiwell plates)

배송 상태

dry ice

저장 온도

−20°C

유전자 정보

human ... CS(1431)

일반 설명

The Citrate Synthase Assay Kit contains all the required reagents (including a positive control enzyme) for a fast and simple measurement of citrate synthase activity in a whole cell extract or in isolated mitochondria. In addition, the kit enables testing of the intactness of the mitochondrial inner membrane.

생화학적/생리학적 작용

Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. The enzyme catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate. This enzyme is an exclusive marker of the mitochondrial matrix.

분석 메모

The activity of the enzyme is measured by following the color of TNB, which is generated from DTNB present in the reaction of citrate synthesis, and caused by the deacetylation of Acetyl-CoA. The overall reaction product, TNB, absorbs at 412 nm.

키트 구성품 전용

제품 번호
설명

  • Assay buffer for citrate synthase 5x 25 mL

  • Bicine buffer 10 mL

  • CelLytic M Cell Lysis Reagent 10 mL

  • DTNB [5,5'-Dithiobis-(2-nitrobenzoic acid)] 4 mg

  • Acetyl coenzyme A 25 mg

  • Oxaloacetic acid 1 g

  • Citrate synthase (positive control) .1 mL

픽토그램

Health hazardExclamation mark

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

10 - Combustible liquids


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