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Merck
모든 사진(1)

주요 문서

D2196

Sigma-Aldrich

DAF-FM solution

5 mM in DMSO, ≥97% (HPLC)

동의어(들):

4-Amino-5-methylamino- 2′,7′-difluorofluorescein, Diaminofluorescein-FM

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About This Item

실험식(Hill 표기법):
C21H14F2N2O5
CAS Number:
Molecular Weight:
412.34
UNSPSC 코드:
12352200
NACRES:
NA.77

Quality Level

분석

≥97% (HPLC)

형태

DMSO solution

저장 조건

desiccated

색상

colorless to yellow, including pink

배송 상태

wet ice

저장 온도

−20°C

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

InChI key

DIJCILWNOLHJCG-UHFFFAOYSA-N

애플리케이션

DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.

생화학적/생리학적 작용

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.

특징 및 장점

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Detection of protein S-nitrosothiols (SNOs) in plant samples on diaminofluorescein (DAF) gels
Rodriguez-Ruiz M, et al.
Bio-protocol null
Marta Rodríguez-Ruiz et al.
Bio-protocol, 7(18), e2559-e2559 (2017-09-20)
In plant cells, the analysis of protein S-nitrosothiols (SNOs) under physiological and adverse stress conditions is essential to understand the mechanisms of Nitric oxide (NO)-based signaling. We adapted a previously reported protocol for detecting protein SNOs in animal systems (

문서

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Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

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