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Merck
모든 사진(1)

주요 문서

D3035

Sigma-Aldrich

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, female

동의어(들):

DNA

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
41106310
NACRES:
NA.52

생물학적 소스

human placenta

Quality Level

Grade

for molecular biology

양식

buffered aqueous solution

배송 상태

dry ice

저장 온도

−20°C

SMILES string

[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

애플리케이션

Deoxyribonucleic acid from human placenta has been used:
  • to compare the efficiency of DNA quantification methods
  • as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines
  • as an internal control to estimate the degree of fragmentation of circulating DNA
  • for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11
  • to compare the 70-bp P5 exon sequence between DNA of different human and primate species
  • to calculate copy number of genes, relative to normal human tissue
  • in PCR reactions
For use in Southern hybridizations.

추천 제품

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

관련 제품

제품 번호
설명
가격

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Alain R Thierry et al.
Nucleic acids research, 38(18), 6159-6175 (2010-05-25)
Although circulating DNA (ctDNA) could be an attractive tool for early cancer detection, diagnosis, prognosis, monitoring or prediction of response to therapies, knowledge on its origin, form and rate of release is poor and often contradictory. Here, we describe an
Joanne S Aveyard et al.
The Journal of molecular diagnostics : JMD, 6(4), 356-365 (2004-10-28)
Many tumors have large homozygous deletions of the CDKN2A locus (encoding p14(ARF) and p16) and of CDKN2B (p15). Our aim was to determine which gene is the major target in bladder cancer. We used quantitative real-time PCR (RTQ-PCR) to determine
Heather Hoover et al.
Journal of proteome research, 14(9), 3670-3679 (2015-07-08)
Tumor types can be defined cytologically by their regions of chromosomal amplification, which often results in the high expression of both mRNA and proteins of certain genes contained within the amplicon. An important strategy for defining therapeutically relevant targets in
Claire E L Smith et al.
PloS one, 12(9), e0185678-e0185678 (2017-09-29)
The imprinted gene PLAGL1 is an important regulator of apoptosis and cell cycle arrest. Loss of its expression has been implicated in tumorigenesis in a range of different cancers, and overexpression during fetal development causes transient neonatal diabetes mellitus (TNDM).
Naoe Kotomura et al.
PloS one, 10(5), e0128282-e0128282 (2015-05-29)
The human CYP19 gene encodes aromatase, which converts androgens to estrogens. CYP19 mRNA variants are transcribed mainly from three promoters. Quantitative RT-PCR was used to measure the relative amounts of each of the three transcripts and determine the on/off state

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