F1757

Fumarase from porcine heart

ammonium sulfate suspension, ≥300 units/mg protein (biuret)

• 동의어(들): L-Malate hydrolyase, Fumarate hydratase
• CAS Number: 9032-88-6
• 효소 위원회 번호: 4.2.1.2 (BRENDA, IUBMB)
• EC Number: 232-887-7
• MDL number: MFCD00131111
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 형태: ammonium sulfate suspension
• Quality Level: 200
• 특이 활성도: ≥300 units/mg protein (biuret)
• 외래 활성: malic dehydrogenase ≤0.1%
• 저장 온도: 2-8°C

설명

일반 설명

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.

Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.

애플리케이션

Fumarase from porcine heart has been used:

• as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
• for immobilized enzyme based stability assay
• in fumarate hydratase assays in human lung carcinoma cells (A549)

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.

생화학적/생리학적 작용

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.

Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.

단위 정의

One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.

물리적 형태

Suspension in 3.2 M (NH₄)₂SO₄, 0.05 M KH₂PO₄, pH 7.5, 0.014 M 2-mercaptoethanol

제조 메모

Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable