G4252

Glyoxalase I from Saccharomyces cerevisiae

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

• 동의어(들): S-Lactoyl-glutathione methylglyoxal-lyase (isomerizing)
• CAS Number: 9033-12-9
• 효소 위원회 번호: 4.4.1.5 (BRENDA, IUBMB)
• MDL number: MFCD00131225
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Grade IV
• Quality Level: 200
• 형태: buffered aqueous glycerol solution
• 특이 활성도: ≥400 units/mg protein
• 외래 활성: glyoxalase II ≤1%
• 저장 온도: 2-8°C

설명

일반 설명

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn²⁺ metalloenzyme.

애플리케이션

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.

생화학적/생리학적 작용

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

단위 정의

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.

물리적 형태

Solution in 50% glycerol, 0.4 M (NH₄)₂SO₄ and 0.002 M KH₂PO₄ pH 6.5

분석 메모

Protein determined by biuret.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)