I0783

Monoclonal Anti-ILK antibody produced in mouse

~2 mg/mL, clone 65.1, purified immunoglobulin, buffered aqueous solution

• 동의어(들): Anti-Integrin-linked protein kinase
• MDL number: MFCD01633545
• UNSPSC 코드: 12352203
• NACRES: NA.44

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 65.1, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~59 kDa
• 종 반응성: rat, human, bovine, monkey, mouse, canine
• 농도: ~2 mg/mL
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.5-2.0 μg/mL using whole cell extract of Chinese hamster ovary cell line (CHO cells)
• 동형: IgG2b
• UniProt 수납 번호: Q13418
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ILK(3611) ; mouse ... Ilk(16202) ; rat ... Ilk(170922)

설명

일반 설명

Monoclonal Anti-ILK (mouse IgG2b isotype) is derived from the 65.1 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from BALB/c mice immunized with purified mouse ILK recombinant protein. Integrin-linked kinase (ILK) is a ubiquitously expressed 50-59 kDa serine/threonine kinase that has three structurally well-conserved domains. A C-terminal domain contains the kinase catalytic site as well as the binding site for integrin β1 cytoplasmic domain. A N-terminal domain contains four ankyrin repeats (ANK).

면역원

purified mouse ILK recombinant protein.

애플리케이션

Monoclonal Anti-ILK antibody produced in mouse has been used in:

• immunostaining
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry
• western blotting

생화학적/생리학적 작용

Integrin-linked kinase (ILK) is a serine-threonine kinase that interacts with PINCH and parvin to modulate cell adhesion, growth, differentiation, migration and invasion. This kinase binds to integrin β1, β2 and β3 domains to regulate integrin signalling. ILK functions as a receptor-proximal effector for integrin and growth factor dependent signal transduction. ILK is associated with cell cycle progression and oncogenic transformation. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-extracellular matrix (ECM) interactions and by certain growth factors. Negative regulation of ILK is mediated by two phosphatases: phosphatase and tensin homolog (PTEN), a tumor suppressor lipid phosphatase and ILKAP (ILK associated serine/threonine phosphatase), a protein phosphatase 2C (PP2C) protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.