L1006

L-Lactic Dehydrogenase from bovine heart

Type XVII, buffered aqueous glycerol solution, ≥400 units/mg protein

• 동의어(들): Lactate, (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD
• CAS Number: 9001-60-9
• 효소 위원회 번호: 1.1.1.27 (BRENDA, IUBMB)
• EC Number: 232-617-8
• MDL number: MFCD00131466
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine heart
• 유형: Type XVII
• 분석: 5.0—15.0 mg protein/mL (biuret)
• 형태: buffered aqueous glycerol solution
• 특이 활성도: ≥400 units/mg protein
• 분자량: 140 kDa
• 제조업체/상표: Sigma-Aldrich
• 기술: cell based assay: suitable
• 불순물: <10 μg/mg protein free ammonium
• 색상: white
• pH: 7.5
• 양이온 미량물: NH₄⁺: ≤10 μg/mg protein
• UniProt 수납 번호: P19858; Q5E9B1
• 응용 분야: life science and biopharma
• 외래 활성: glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase ≤0.02%; pyruvate kinase, myokinase and α-glycerophosphate dehydrogenase ≤0.01%
• 저장 온도: 2-8°C
• 유전자 정보: cow ... LDHA(281274) , LDHB(281275)

설명

일반 설명

Research area: Cell signaling. L-LacticDehydrogenase (LDH) is present in the cell cytoplasm. and is part of the glycolytic pathway. Lactate dehydrogenase (LDH) is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family. It has five isozymes (LD₁ to LD₅) and this composition varies in different tissues. LD₁ is present at higher concentrations in the heart, kidneys, and erythrocytes. LD₅ is found in the liver and skeletal muscles.(2)

애플리케이션

L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca²⁺-ATPase (SERCA).

생화학적/생리학적 작용

Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis. Dye based purified LDH from bovine heart has analytical applications.Lactic dehydrogenase (LDH)plays an essential role in the glycolytic pathway where it is involved in the conversionof pyruvate to lactate using NAD⁺as a co-factor. It is considered an important molecule that can be used incancer therapy as it acts as a glycolytic inhibitor. Inhibition of LDH isassociated with blocking of aerobic glycolysis in the tumour cells.

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

단위 정의

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

물리적 형태

Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5

분석 메모

Protein determined by biuret

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1