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Merck
모든 사진(1)

주요 문서

L8402

Sigma-Aldrich

Lysozyme from human neutrophils

≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)

동의어(들):

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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About This Item

CAS Number:
효소 위원회 번호:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

human neutrophils

Quality Level

재조합

expressed in E. coli

분석

≥95% (SDS-PAGE)

양식

lyophilized powder

특이 활성도

≥100,000 units/mg protein (E1%/280)

기술

cell based assay: suitable

적합성

suitable for cell lysis

UniProt 수납 번호

응용 분야

cell analysis

저장 온도

−20°C

SMILES string

N1([C@@H](CCC1)C(=O)N[C@@H](CCC(=O)N)C(=O)NCC(=O)N[C@@H]([C@H](CC)C)C(=O)N[C@@H](CCCN\C(=N\[H])\N)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc4c5c([nH]c4)cccc5)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc2c3c([nH]c2)cccc3)C(=O)N[C@@H](CCCN\C(=N\[H])\N)C(=O)N[C@@H]

InChI

1S/C99H159N37O23/c1-11-50(8)77(132-72(139)46-120-81(145)63(32-33-70(101)137)124-88(152)69-31-21-39-136(69)93(157)68(43-73(140)141)131-84(148)62(29-19-37-116-98(109)110)125-90(154)75(48(4)5)135-91(155)76(49(6)7)133-80(144)57(100)24-16-34-113-95(103)104)92(156)126-60(27-17-35-114-96(105)106)82(146)121-51(9)78(142)129-66(41-54-45-119-59-26-15-13-23-56(54)59)87(151)134-74(47(2)3)89(153)122-52(10)79(143)128-65(40-53-44-118-58-25-14-12-22-55(53)58)85(149)123-61(28-18-36-115-97(107)108)83(147)130-67(42-71(102)138)86(150)127-64(94(158)159)30-20-38-117-99(111)112/h12-15,22-23,25-26,44-45,47-52,57,60-69,74-77,118-119H,11,16-21,24,27-43,46,100H2,1-10H3,(H2,101,137)(H2,102,138)(H,120,145)(H,121,146)(H,122,153)(H,123,149)(H,124,152)(H,125,154)(H,126,156)(H,127,150)(H,128,143)(H,129,142)(H,130,147)(H,131,148)(H,132,139)(H,133,144)(H,134,151)(H,135,155)(H,140,141)(H,158,159)(H4,103,104,113)(H4,105,106,114)(H4,107,108,115)(H4,109,110,116)(H4,111,112,117)/t50-,51-,52-,57-,60-,61-,62-,63-,64-,65-,66-,67-,68-,69-,74-,75-,76-,77-/m0/s1

InChI key

ZJCXKXFAOCLSRV-RYLVIXIQSA-N

유전자 정보

human ... LYZ(4069)

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일반 설명

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.

애플리케이션

Lysozyme from human neutrophils has been used in the checkerboard method for establishing a synergy between peptidomimetic 1 and immune effector molecules against E. coli.

생화학적/생리학적 작용

Lysozymes participates in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

단위 정의

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

물리적 형태

Lyophilized from 50 mM sodium acetate, pH 6.0, with 100 mM NaCl

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

María Victoria Humbert et al.
PLoS pathogens, 13(6), e1006448-e1006448 (2017-07-01)
Pathogenic and commensal Neisseria species produce an Adhesin Complex Protein, which was first characterised in Neisseria meningitidis (Nm) as a novel surface-exposed adhesin with vaccine potential. In the current study, the crystal structure of a recombinant (r)Nm-ACP Type I protein
Maj Hedtjärn et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 24(12), 1333-1351 (2004-12-31)
Brain ischemia triggers an inflammatory reaction that progresses for days to weeks and seems to have a role in secondary progression of injury. Inflammation induces a complex pattern of signaling molecules with partly contradictory actions, and the responses may be
Selectivity in the potentiation of antibacterial activity of alpha-peptide beta-peptoid peptidomimetics and antimicrobial peptides by human blood plasma
Hein-Kristensen L, et al.
Research in Microbiology, 164(9), 933-940 (2013)
Natural Food Antimicrobial Systems (2000)
Leonid Breydo et al.
Biochimica et biophysica acta, 1844(2), 346-357 (2013-11-21)
The intracellular environment contains high concentrations of macromolecules occupying up to 30% of the total cellular volume. Presence of these macromolecules decreases the effective volume available for the proteins in the cell and thus increases the effective protein concentrations and

프로토콜

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

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