NA0600

GenElute^™ HP Endotoxin-Free Plasmid Megaprep Kit

1 kit sufficient for 5 preparations

• 동의어(들): Gen Elute, GenElute Endotoxin-Free Plasmid Kit
• UNSPSC 코드: 41105501
• NACRES: NA.52

속성

• 사용: kit sufficient for 5 preparations
• 환경친화적 대안 제품 특성: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 환경친화적 대안 카테고리: , Aligned
• 저장 온도: 15-25°C

설명

일반 설명

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

The GenElute HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 5 mg of high copy plasmid DNA with ≤ 0.1 endotoxin units/μg can be recovered from 200 mL - 1.0 L LB medium or 200 - 600 mL TB medium of E. coli culture in less than 1.5 hours. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digest, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

애플리케이션

GenElute^™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

특징 및 장점

• Up to 5 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
• Only 90 minutes from pelleted cells to purified plasmid
• Vacuum format with no ethanol precipitation required

기타 정보

For additional information, please see www.sigma-aldrich.com/genelutehp.

법적 정보

GenElute is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• 픽토그램: GHS02,GHS08,GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H226,H290,H302 + H332,H315,H319,H334,H336,H372
• 예방조치 성명서: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
• 표적 기관: Central nervous system, Lungs
• Storage Class Code: 3 - Flammable liquids
• Flash Point (°F): 77.0 °F - closed cup
• Flash Point (°C): 25 °C - closed cup