NUC101

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)

• 동의어(들): Rapid mammalian nuclei isolation kit
• UNSPSC 코드: 12352207
• NACRES: NA.32

속성

• 사용: sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)
• Quality Level: 200
• 유통기한: 1 yr at 2‑8 °C
• 포장: pkg of 1 kit
• 응용 분야: cell analysis
• 외래 활성: nuclease and protease, free
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

애플리케이션

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

생화학적/생리학적 작용

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

기타 정보

All procedures should be carried out on ice or 2-8°C.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable