추천 제품
Quality Level
사용
kit sufficient for 10 extractions (1 ml packed cell volume)
kit sufficient for 100 extractions (100 μl packed cell volume)
기술
protein extraction: suitable
western blot: suitable
배송 상태
dry ice
저장 온도
−20°C
일반 설명
The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.
애플리케이션
NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.
기타 정보
Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.
법적 정보
NuCLEAR is a trademark of Sigma-Aldrich Co. LLC
키트 구성품 역시 별도로 이용 가능함
제품 번호
설명
SDS
- 3× Dilution and Equilibration Buffer 90 mL
- P8340Protease Inhibitor Cocktail 1 mLSDS
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point (°F)
188.6 °F - closed cup
Flash Point (°C)
87 °C - closed cup
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이미 열람한 고객
F Guidez et al.
Molecular and cellular biology, 18(7), 3851-3861 (1998-06-25)
Granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) independently stimulate the proliferation and differentiation of macrophages from bone marrow progenitor cells. Although the GM-CSF and M-CSF receptors are unrelated, both couple to Ras-dependent signal transduction pathways, suggesting that these
K A Lee et al.
Gene analysis techniques, 5(2), 22-31 (1988-03-01)
A convenient and rapid method for preparing soluble extracts from the nuclei of as few as 3 x 10(7) mammalian cells (miniextract procedure) is described. By several criteria, miniextracts are comparable to nuclear extracts prepared from large numbers of cells
Isolation of intact nuclei for nuclear extract preparation from a fragile B-lymphocyte cell line.
R B Dyer et al.
BioTechniques, 19(2), 192-195 (1995-08-01)
Fangyuan Dong et al.
EBioMedicine, 39, 472-483 (2018-12-12)
Accumulating evidence has revealed the pivotal role of epigenetic regulation in the pathogenesis of liver disease. However, the epigenetic mechanism that accounts for hepatic stellate cells (HSCs) activation in liver fibrosis remains largely unknown. Primary HSCs were used to screen
Qing Wang et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 302(9), R1025-R1033 (2012-03-10)
We previously reported that excess of deoxycorticosterone-acetate (DOCA)/salt-induced cardiac hypertrophy in the absence of hypertension in one-renin gene mice. This model allows us to study molecular mechanisms of high-salt intake in the development of cardiovascular remodeling, independently of blood pressure
문서
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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