P3201

Platelet-Derived Growth Factor-BB human

≥98% (HPLC and SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): PDGF-BB
• MDL number: MFCD00213023
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• product name: Platelet-Derived Growth Factor-BB human, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥98% (HPLC and SDS-PAGE)
• 형태: lyophilized powder
• 효능: 1.0-3.0 ng/mL EC₅₀
• 품질: endotoxin tested
• 분자량: ~25 kDa
• 포장: pkg of 10 μg; pkg of 50 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/μg
• UniProt 수납 번호: P01127
• 저장 온도: −20°C
• 유전자 정보: human ... PDGFB(5155)

설명

일반 설명

Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved mapped to human chromosome 7p22.3. The active form of Pdgf exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.

애플리케이션

Platelet-Derived Growth Factor-BB human has been used :

• As a growth supplement in proximal tubule and glomerular explant cell culture
• In three-dimensional scaffold preparation
• To study the effect of brivanib on growth factor signaling pathways
• In transwell migration assay

생화학적/생리학적 작용

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF significantly activates chemotaxis of neutrophils, monocytes, and fibroblasts. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.

기타 정보

Dimer of the B chain of human PDGF.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in 30 mM acetic acid.

분석 메모

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)