추천 제품
Grade
Molecular Biology
for molecular biology
양식
buffered aqueous glycerol solution
분자량
~80 kDa
농도
≥10,000 units/mL
UniProt 수납 번호
외래 활성
DNase, RNase, none detected
배송 상태
wet ice
저장 온도
−20°C
유전자 정보
cow ... ALPI(280993)
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일반 설명
Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.
애플리케이션
Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.
성분
Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.
단위 정의
One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.
기타 정보
Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.
관련 제품
제품 번호
설명
가격
Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
기질
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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프로토콜
CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
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