추천 제품
생물학적 소스
rabbit
Quality Level
결합
unconjugated
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
형태
buffered aqueous solution
분자량
antigen 57 kDa
종 반응성
human, bovine, rat, mouse
향상된 검증
independent
Learn more about Antibody Enhanced Validation
기술
immunoprecipitation (IP): 2-5 μg using RIPA lysate (500 μg) of rat NRK cells
indirect immunofluorescence: 2-5 μg/mL using human HeLa cells
western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of mouse NIH3T3 cells
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... P4HB(5034)
mouse ... P4hb(18453)
rat ... P4hb(25506)
일반 설명
Anti-Protein Disulfide Isomerase (PDI) (MD-12) is developed in rabbit using as immunogen a synthetic peptide corresponding to amino acid residues of human PDI with N-terminal added cysteine, conjugated to KLH. Protein disulfide isomerase (PDI, Erp58) is an abundant multifunctional, soluble enzyme (E.C. 5.3.4.1) that resides in the lumen of the endoplasmic reticulum of eukaryotic cells. The mammalian PDI family comprises several highly divergent proteins that contain one or more thioredoxin like structural domains. PDI consists of four tandem domains, two of which contain a catalytic site for S-S bond formation. PDI has an N-terminal ER signal and C-terminal ER retention KDEL signal sequences. PDI may also be expressed in other cellular localizations such as the cell surface, cytosol, and nucleus. PDI was found on the cell surface of several cell types including platelets, lymphoid cells, pancreatic exocrine cells, retinal cells, thyroid cells, and hepatocytes.
면역원
synthetic peptide corresponding to amino acid residues 495-506 of human protein disulfide isomerase.
애플리케이션
Anti-Protein Disulfide Isomerase (MD-12) antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
생화학적/생리학적 작용
Protein disulfide isomerase (PDI) catalyses the formation and rearrangements of both intrachain and interchain disulfide bonds in secreted proteins. PDI also serves as a molecular chaperone that can suppress protein aggregation, or as an anti-chaperone that mediates aggregate formation. PDI respectively participates in the hydroxylation of prolines in procollagen during collagen synthesis and in the transfer of neutral lipid onto nascent lipoprotein particles. PDI has calcium-dependent transglutaminase activity, which catalyses the formation of isopeptide bonds.
물리적 형태
Solution in 0.01 M phosphate buffered saline containing 1% bovine serum abumin and 15 mM sodium azide.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Xinmiao Fu et al.
The Biochemical journal, 447(1), 115-123 (2012-07-04)
E(2) (17β-oestradiol), a female sex hormone, has important biological functions in a woman's body. The pancreas, often considered a non-classical E(2)-targeting organ, is known to be functionally regulated by E(2), but little is known about how oestrogen actions are regulated
Formation of transitory intrachain and interchain disulfide bonds accompanies the folding and oligomerization of simian virus 40 Vp1 in the cytoplasm
Li PP, et al.
Proceedings of the National Academy of Sciences of the USA, 99(3), 1353-1358 (2002)
Hongge Wang et al.
Molecular and cellular biology, 42(6), e0052221-e0052221 (2022-05-03)
Ferroptosis is a form of regulated cell death resulting predominantly from catastrophic accumulation of lipid reactive oxygen species (ROS). While the antioxidant systems that counter ferroptosis have been well characterized, the mechanism underlying ferroptosis-associated accumulation of lipid ROS remains unclear.
Protein disulfide isomerase. A multifunctional protein resident in the lumen of the endoplasmic reticulum.
Noiva R and Lennarz WJ
The Journal of Biological Chemistry, 267(6), 3553-3556 (1992)
Dual targeting of the protein disulfide isomerase RB60 to the chloroplast and the endoplasmic reticulum
Levitan A, et al.
Proceedings of the National Academy of Sciences of the USA, 102(17), 6225-6230 (2005)
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