P9249

Monoclonal Anti-p53−Biotin antibody produced in mouse

~1 mg/mL, clone DO-1, ascites fluid, buffered aqueous solution

• MDL number: MFCD01864735
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: biotin conjugate
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: DO-1, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~53 kDa
• 종 반응성: human
• 농도: ~1 mg/mL
• 기술: flow cytometry: suitable; immunocytochemistry: 5-10 μg/mL using human A431; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1 μg/mL using human A431 cell extracts
• 동형: IgG2a
• UniProt 수납 번호: P04637
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... TP53(7157)

설명

일반 설명

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

면역원

recombinant human wild type p53. The epitope resides beween amino acids 20-25 of human p53.

애플리케이션

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

생화학적/생리학적 작용

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2-8°C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree”freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

기타 정보

In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)