PLN350
GenElute™ Plasmid Miniprep Kit
sufficient for 350 purifications
동의어(들):
GenElute™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute
사용
sufficient for 350 purifications
Quality Level
환경친화적 대안 제품 특성
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
기술
DNA purification: suitable
환경친화적 대안 카테고리
저장 온도
15-25°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
애플리케이션
The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.
The kit has been used for the purification of plasmid DNA of Dirofilaria repens.
특징 및 장점
- Purified plasmid DNA in less than 30 minutes
- Purify up to 15 μg of plasmid DNA
- No detectable genomic DNA or RNA contamination
- No phenol/chloroform extraction or alcohol precipitation required
기타 정보
For additional information, please see www.sigma-aldrich.com/genelutehp.
법적 정보
GenElute is a trademark of Sigma-Aldrich Co. LLC
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 기관
Central nervous system
Storage Class Code
3 - Flammable liquids
Vanessa Meier-Stephenson et al.
The Journal of biological chemistry, 296, 100589-100589 (2021-03-29)
Approximately 250 million people worldwide are chronically infected with the hepatitis B virus (HBV) and are at increased risk of developing cirrhosis and hepatocellular carcinoma. The HBV genome persists as covalently closed circular DNA (cccDNA), which serves as the template
Simeon S Andrews et al.
The Journal of biological chemistry, 294(30), 11549-11558 (2019-06-12)
Two-hybrid systems can be used for investigating protein-protein interactions and may provide important information about gene products with unknown function. Despite their success in mapping protein interactions, two-hybrid systems have remained mostly untouched by improvements in next-generation DNA sequencing. The
Dirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports).
Mateju J
BMC Infectious Diseases, 16, 171-171 (2016)
Richard A Urbanowicz et al.
Cell, 167(4), 1079-1087 (2016-11-05)
The 2013-2016 outbreak of Ebola virus (EBOV) in West Africa was the largest recorded. It began following the cross-species transmission of EBOV from an animal reservoir, most likely bats, into humans, with phylogenetic analysis revealing the co-circulation of several viral
Mahmood Faraz et al.
The Journal of biological chemistry, 293(9), 3421-3435 (2018-01-11)
Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) is a tumor suppressor and a negative regulator of several receptor tyrosine kinases. The molecular mechanisms by which LRIG1 mediates its tumor suppressor effects and regulates receptor tyrosine kinases remain incompletely understood. Here
관련 콘텐츠
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자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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