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Merck
모든 사진(1)

Key Documents

SRP2015

Sigma-Aldrich

RNA Polymerase II, p14.5 subunit human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

동의어(들):

RPB9, hRPB14.5

로그인조직 및 계약 가격 보기

크기 선택

1 EA
₩1,761,939

₩1,761,939


재고 정보

예상 입고일2025년 2월 17일세부사항



크기 선택

보기 변경
1 EA
₩1,761,939

About This Item

UNSPSC 코드:
12352202
NACRES:
NA.26

₩1,761,939


재고 정보

예상 입고일2025년 2월 17일세부사항


생물학적 소스

human

재조합

expressed in E. coli

분석

≥80% (SDS-PAGE)

형태

frozen liquid

분자량

~16.6 kDa

포장

pkg of 10 μg

저장 조건

avoid repeated freeze/thaw cycles

농도

300 μg/mL

기술

western blot: suitable

색상

clear colorless

생화학적/생리학적 작용

hRPB9 is a subunit unique to RNA polymerase II, although it has sequence homologues in RNA polymerases I and III. The gene for Rpb9 is not essential for yeast cell viability, but is essential in Drosophila. hRPB9 has roles in both transcription initiation and transcription elongation. In the initiation reaction it is necessary for accurate start site selection. In the elongation reaction, RPB9, along with TFIIS facilitates the conversion of an arrest-competent conformation to a read-through competent conformation. RNA polymerase II lacking the RPB9 subunit uses alternate transcription initiation sites in vitro and in vivo and is unable to respond to the transcription elongation factor TFIIS in vitro. A role in the modulation of initiation and elongation is consistent with the localization of RPB9 in the three-dimensional structure of yeast RNA polymerase II. RPB9 is located at the tip of the so-called "jaws" of the enzyme, which is thought to function by clamping the DNA downstream of the active site. RPB9 comprises two zinc ribbon domains joined by a conserved linker region. The C-terminal zinc ribbon is similar in sequence to that found in TFIIS.

물리적 형태

Clear and colorless frozen liquid solution

제조 메모

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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    문서 라이브러리 방문

    Harrison, D.A., et al.
    Molecular and Cellular Biology, 11, 928-935 (1992)
    Y Nogi et al.
    Molecular and cellular biology, 13(1), 114-122 (1993-01-01)
    We have previously isolated mutants of Saccharomyces cerevisiae that are primarily defective in transcription of 35S rRNA genes by RNA polymerase I and have identified genes (RRN1 to RRN9) involved in this process. We have now cloned the RRN4 gene
    N A Woychik et al.
    The Journal of biological chemistry, 266(28), 19053-19055 (1991-10-05)
    The Saccharomyces cerevisiae RNA polymerase II subunit gene RPB9 was isolated and sequenced. RPB9 is a single copy gene on chromosome VII. The RPB9 sequence predicts a protein of 122 amino acids with a molecular mass of 14,200 Da. The

    자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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